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首頁(yè) /藥靶模型 /GPCR靶點(diǎn)細(xì)胞 /Orphan and other 7TM /mMRGPRB2/Gα15/HEK293

mMRGPRB2/Gα15/HEK293

CBP71341

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I. Background

Mast cell-specific receptor for basic secretagogues, i.e. cationic amphiphilic drugs, as well as endo- or exogenous peptides, consisting of a basic head group and a hydrophobic core. Recognizes and binds small molecules containing a cyclized tetrahydroisoquinoline (THIQ), such as non-steroidal neuromuscular blocking drugs (NMBDs), including tubocurarine and atracurium. In response to these compounds, mediates pseudo-allergic reactions characterized by histamine release, inflammation and airway contraction.

 
II. Introduction
Host Cell:

HEK293

Stability: 20 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Freeze Medium: 90% FBS+10% DMSO
Culture Medium: DMEM+10%FBS+400ug/ml G418+ 100 μg/ml Hygromycin B
Mycoplasma Status: Negative
Storage: Liquid nitrogen immediately upon delivery
Application(s):

Functional assay for mMRGPRB2

Transducer: Gq
 
Ⅲ. Description of Host Cell Line
Organism: Human
Tissue: kidney
Morphology: Epithelial
Growth Properties: Adherent
 
Ⅳ. Representative Data

Figure 1. mMRGPRB2/Gα15/HEK293, the EC50 was 1.47μg/ml.


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